Publications & Resources

Publications (2022 - current)

2026

Bhowmik, P.K., Williams, J.T., Polley, B., Chen, N., Kavuri, N.R., Zang, W., Barakate, A., Yang, H., Narra, M.K., Beattie, A.D., Starker, C., Voytas, D.F. and Baysal, C. (2026) BSMV-mediated genome editing exhibits host-specific heritability: germline transmission in barley and somatic edits in Nicotiana benthamiana. BMC Plant Biology.
 
Jiang, H., Myers, E.A., Millman, B., Lee, D.-Y., Nusinow, D.A., Voytas, D.F. and Baxter, I. (2026) Development of male-sterile lines of Setaria viridis to accelerate C4 model plant genetics. The Plant Journal: For Cell and Molecular Biology, 126(2), p. e70880. 
 

Tibebu R, Gamo ME, Ellison EE, Myers EA, Sahoo R, Winecke SR, Tan, GD, Fischer JM, Leakey ADB, Voytas DF. (2026) Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics. The Plant Journal, 125(6), p. e70805.

Tibebu R, Ellison EE, Winecke SR, Prichard LE, Klejeski N, Donahue LI, Cody JP, Baysal C, Starker CG, Van Eck J, Voytas DF. (2026) Virus-mediated, heritable gene editing in groundcherry (Physalis grisea). Frontiers in Plant Science, Volume 17-2026.

 

2025

Butler NM, Carlson AT, Starker C, Voytas DF (2025) Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.). Plant Biotechnology Journal.
 

Chamness, J. C.; Cody, J. P.; Cruz, A. J.; Voytas, D. F. Viral delivery of recombinases activates heritable genetic switches in plants. (2025) Plant Physiology. 

Marín‐Sanz, M.; Sánchez‐León, S.; Guzmán‐López, M. H.; Starker, C. G.; Voytas, D. F.; Barro, F. Cas9‐ and Cas12a‐mediated excision and replacement of the celiac disease‐related α‐gliadin immunogenic complex in hexaploid wheat. (2025) Plant Biotechnology Journal, pbi.70200. 

Sajid SN, Chen W, Cody JP, He L, Steinberger AR, Wei F, Xie Z, Tian B, Voytas DF, Shi G (2025) Agrobacterium-mediated root organogenesis and open treatment system (AgroROOTs) for root functional genomics studies in cotton. Industrial Crops and Products, 236:122112. 

Steinberger, A. R.; Voytas, D. F. Virus-induced gene editing free from tissue culture. (2025) Nature Plants. 

Wang, L.; O’Conner, S.; Tanvir, R.; Zheng, W.; Cothron, S.; Towery, K.; et al. CRISPR/Cas9-based editing of NF-YC4 promoters yields high-protein rice and soybean. (2025) The New Phytologist, 245(5), 2103–2116. 

Zinselmeier, M. H.; Casas-Mollano, J. A.; Cors, J.; Ferreira, S. S.; Voytas, D. F.; Smanski, M. J. Towards engineering hybrid incompatibility in plants. (2025) Plant Biotechnology Journal, 23: 2752-2754.

Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF. Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery. (2024) The Plant Journal. 121(2), p. e17196.

 

 

2024

Liu D, Myers EA, Xuan S, Prichard LE, Donahue LI, Ellison EE, Starker CG, Voytas DF. Heritable, multinucleotide deletions in plants using viral delivery of a repair exonuclease and guide RNAs. (2024) Plant Physiol, 194(4):2229-2239. doi: 10.1093/plphys/kiae015. PMID: 38243587.

Zinselmeier MH, Casas-Mollano JA, Cors J, Sychla A, Heinsch SC, Voytas DF, Smanski MJ. Optimized dCas9 programmable transcriptional activators for plants. (2024) Plant Biotechnol J. 

Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, Prichard LE, Starker CG, Voytas DF. Heritable gene editing in tomato through viral delivery of isopentenyl transferase and single-guide RNAs to latent axillary meristematic cells. (2024) Proc Natl Acad Sci USA, 121(39):e2406486121. 

 

2023

Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R, Gamo ME, Starker CG, Zhang F, Voytas DF. An extensible vector toolkit and parts library for advanced engineering of plant genomes. (2023) Plant Genome, e20312. 

Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF. Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana. (2023) Nat Protoc, 18(1):81-107. 

Yu, Y., Hu, H., Voytas, D.F., Doust, A.N. and Kellogg, E.A. The YABBY gene SHATTERING1 controls activation rather than patterning of the abscission zone in Setaria viridis. (2023) New Phytol, 240: 846-862.

 

2022

Degao Liu, Shuya Xuan, Lynn E Prichard, Lilee Donahue, Changtian Pan, Ugrappa Nagalakshmi, Evan E Ellison, Colby G Starker, Savithramma P Dinesh-Kumar, Yiping Qi, Daniel F Voytas, Heritable base-editing in Arabidopsis using RNA viral vectors. (2022) Plant Physiology.

Nagalakshmi U, Meier N, Liu JY, Voytas DF, Dinesh-Kumar SP. High-efficiency multiplex biallelic heritable editing in Arabidopsis using an RNA virus. (2022) Plant Physiology.

 

Resources

 

Genome Engineering Toolkit

  • Unfortunately, the Voytas Lab Genome Engineering Toolkit is inactive until further notice. This zip file contains all plasmid maps and protocols from the Cermak, et al., 2017 publication. All primer design is outlined in the protocols. If you have questions, please contact Colby Starker at <[email protected]>.
     

AddGene

  • A nonprofit global plasmid repository. They archive and distribute plasmids for the Voytas lab: Voytas TALEN Kit
     

Zinc Finger Database